Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription

1 1Society of Fellows, Harvard University, Cambridge, Massachusetts, USA. 2Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. 3Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. 4Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA. 5Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA. 6Center for Regenerative Medicine and Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. 7Present address: Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; McGovern Institute for Brain Research, MIT, Cambridge, Massachusetts, USA; and Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts, USA. 8These authors contributed equally to this work. Correspondence should be addressed F.Z. ([email protected]) or P.A. ([email protected]).